Biologist Nick Bos tells Wikinews about ‘self-medicating’ ants

Tuesday, September 1, 2015

Nick Bos, of the University of Helsinki, studies “the amazing adaptations social insects have evolved in order to fight the extreme parasite pressure they experience”. In a recently-accepted Evolution paper Bos and colleagues describe ants appearing to self-medicate.

The team used Formica fusca, an ant species that can form thousand-strong colonies. This common black ant eats other insects, and also aphid honeydew. It often nests in tree stumps or under rocks and foraging workers can sometimes be spotted climbing trees.

Some ants were infected with Beauveria bassiana, a fungus. Infected ants chose food laced with toxic hydrogen peroxide, whereas healthy ants avoided it. Hydrogen peroxide reduced infected ant fatalities by 15%, and the ants varied their intake depending upon how high the peroxide concentration was.

In the wild, Formica fusca can encounter similar chemicals in aphids and dead ants. The Independent reported self-medicating ants a first among insects.

Bos obtained his doctorate from the University of Copenhagen. He began postdoctoral research at Helsinki in 2012. He also runs the AntyScience blog. The blog aims to help address “a gap between scientists and ‘the general public’.” The name is a pun referencing ants, its primary topic, science, and “non-scientific” jargon-free communication. He now discusses his work with Wikinews.

((Wikinews)) What first attracted you to researching ants?

Nick Bos Me and a studymate were keeping a lot of animals during our studies, from beetles, to butterflies and mantids, to ants. We had the ants in an observation nest, and I could just look at them for hours, watching them go about. This was in my third year of Biology study I think. After a while I needed to start thinking about an internship for my M.Sc. studies, and decided to write a couple of professors. I ended up going to the Centre for Social Evolution at the University of Copenhagen where I did a project on learning in Ants under supervision of Prof. Patrizia d’Ettorre. I liked it so much there I ended up doing a PhD and I’ve been working on social insects ever since.

((Wikinews)) What methods and equipment were used for this investigation?

NB This is a fun one. I try to work on a very low budget, and like to build most of the experimental setups myself (we actually have equipment in the lab nicknamed the ‘Nickinator’, ‘i-Nick’ and the ‘Nicktendo64’). There’s not that much money in fundamental science at the moment, so I try to cut the costs wherever possible. We collected wild colonies of Formica fusca by searching through old tree-trunks in old logging sites in southern Finland. We then housed the ants in nests I made using Y-tong [aerated concrete]. It’s very soft stone that you can easily carve. We carved out little squares for the ants to live in (covered with old CD covers to prevent them escaping!). We then drilled a tunnel to a pot (the foraging arena), where the ants got the choice between the food with medicine and the food without.

We infected the ants by preparing a solution of the fungus Beauveria bassiana. Afterwards, each ant was dipped in the solution for a couple of seconds, dried on a cloth and put in the nest. After exposing the ants to the fungus, we took pictures of each foraging arena three times per day, and counted how many ants were present on each food-source.

This gave us the data that ants choose more medicine after they have been infected.

The result that healthy ants die sooner when ingesting ROS [Reactive Oxygen Species, the group of chemicals that includes hydrogen peroxide] but infected ants die less was obtained in another way (as you have to ‘force feed’ the ROS, as healthy ants, when given the choice, ignore that food-source.)

For this we basically put colonies on a diet of either food with medicine or without for a while. And afterwards either infected them or not. Then for about two weeks we count every day how many ants died. This gives us the data to do a so-called survival analysis.

We measured the ROS-concentration in the bodies of ants after they ingested the food with the medicine using a spectrophotometer. By adding certain chemicals, the ROS can be measured using the emission of light of a certain wave-length.

The detrimental effect of ROS on spores was easy to measure. We mixed different concentrations of ROS with the spores, plated them out on petridishes with an agar-solution where fungus can grow on. A day after, we counted how many spores were still alive.

((Wikinews)) How reliable do you consider your results to be?

NB The results we got are very reliable. We had a lot of colonies containing a lot of ants, and wherever possible we conducted the experiment blind. This means the experimenter doesn’t know which ants belong to which treatment, so it’s impossible to influence the results with ‘observer bias’. However, of course this is proof in just one species. It is hard to extrapolate to other ants, as different species lead very different lives.

((Wikinews)) Where did the ants and fungus you used come from? How common are they in the wild?

NB For ants, see above about the collection.

This species of fungus does appear in Finland, but we chose to use a different strain from Denmark (with thanks to Prof. J. Eilenberg and the laboratory technician Louise Lee Munch Larsen from the University of Copenhagen). Animals can adapt to local strains (‘local adaptation’), and just to make sure we thought it would be good to use a strain of fungus that the ants definitely did not evolve specific resistances against. This means that the reaction of the ants (to self-medicate) is very likely to be a general response, and not just against their local fungal enemies.

((Wikinews)) Are there any ethical considerations around exposing ants to toxins and parasites?

NB Legally, no. Insects do not have any ‘rights’ as such regarding ethics. That said, we do take measures to not make them ‘suffer unnecessarily’. For example, dissections are done when the ants are anesthetized (either by CO2 or Ice), and when ants need to be killed, we do it in alcohol, which kills the ants in a matter of seconds. So while the ants do not have ‘rights’ as such, we still try to handle them with as much respect as possible (even though the experiment involves infecting them with a deadly fungus).

But even though the 12,000 ants in our study sounds like a lot (and it is), this is negligible in the ‘grand scheme of things’. It has been calculated that in the Netherlands alone, nearly a trillion insects die against just the licence-plates of cars every six months. I don’t own a car, so that means I’m excused right? 😉

((Wikinews)) This is the first evidence for self-medicating insects. How widespread do you think this phenomenon could be in reality?

NB It’s not actually the first evidence for self-medication in insects. Moths and fruit flies definitely do it, and there’s evidence in honey bees and bumble-bees as well. So it seems to be quite wide-spread in the insect world. I have no doubt that as time goes on, there will be more and more cases documented. Insects (and animals in general) seem to be quite good at taking care of themselves.

((Wikinews)) How might ants locate healing substances in the wild?

NB Very good question. This is something that’s important to know. If they would only do it in the lab, the behaviour wouldn’t be very interesting. We have some guesses where they might get it from, but at the moment we don’t know yet. That said, I plan to investigate this question (among others) further [in] the next couple of years.

((Wikinews)) For your PhD you researched ants’ scent-based communications. Could healthy ants perhaps tell other ants are infected and encourage this behaviour?

NB There’s not much known about this. There’s conflicting evidence about whether sick ants actually smell different from healthy ones or not. At the moment it seems that sick ants mostly take care of the problem themselves. Sick ants stop most interaction with nestmates and especially brood, and leave the nest to die in isolation. This is probably for reducing chance of infecting nestmates, but of course it also reduces the work load of their nest-mates, as their corpse doesn’t have to be dragged out etc.

So as an answer to the question, I would find it unlikely that such a behaviour would evolve, but it’s not known yet.

((Wikinews)) Ants generally avoided the peroxide if they were healthy, but in some circumstances might they try to build resistance against infection in advance?

NB Who knows? Also not known yet unfortunately. That said, there is a very interesting study about resin collection in ants. Wood ants collect tree-resin, which has anti-microbial properties. They collect this even if not infected, and when you infect them, they don’t collect more of the resin than normal. So basically it seems like they collect it in order to keep diseases out of the nest, so they stop the disease before it can actually infect them.

((Wikinews)) Are there plans to follow this research up? Might you research other species? Other substances?

NB I first want to find out where they get it from in nature. There might be many sources of medicine (recent evidence suggests that tobacco plays a similar role for bumble bees). Dalial Freitak, who is also on this paper is currently running tests with Ph.D. student Siiri Fuchs (who is also on the paper) with other substances to see if any have the same effect as H2O2 [hydrogen peroxide].

Once the behaviour has been well described in this species of ant, I might do a comparison with other species. For example, once we find the source of the medicine in nature… would species without access to this source also have evolved the same behaviour in the lab? And if so… where would they get it from?

Also… can ants medicate their friends? 🙂

((Wikinews)) What other research are you working on right now?

NB Phew…lots! 🙂

I still have some questions left unanswered from my Ph.D. work related to how ants recognize who is a friend and who isn’t. I also started collaborating with Prof. Michael Poulsen from the University of Copenhagen on immunity in fungus-growing termites, as well as their chemical recognition abilities. Furthermore we’re working on social parasitism in wood-ants (ants have lots of animals exploiting the nest for shelter and resources, which all somehow have to get in to the fortress without getting killed).

Thursday, November 18, 2004

In the United Kingdom, the music video for this Christmas’ remake of the charity single Do They Know It’s Christmas? was launched simultaneously today (Thursday) on several UK and Irish television channels including BBC One, BBC Two, RTE 1, ITV1, Channel 4, and Sky One. The debut was made at 17:55 GMT.

The song was originally recorded in 1984 by several of the biggest artists of the time including Bono, Bob Geldof, Paul McCartney and George Michael. This year, in celebration of 20 years since the first single, the song has been remade by over 50 of today’s best selling artists, including Coldplay frontman Chris Martin, Robbie Williams, Bono, Justin Hawkins of The Darkness and Dido.

The song, which is in aid of charity, is already tipped to be the Christmas No. 1 in the UK and Ireland. The song will also be launched as a charity mobile phone ringtone to raise funds for Sudan’s troubled Darfur region. Mycokemusic.com said it was going to put the track on its website from Thursday with all profits to be donated to charity. The single will be available to buy in shops from the 29 November.

Sunday, November 8, 2009

Federal investigators with the Chemical Safety Board (CSB) of the United States have declared that a recent explosion that damaged homes alongside a Woods Cross, Utah refinery was triggered when a pipe burst. The revelation comes as locals begin questioning the decision to build houses near the facility.

The explosion at the Silver Eagle refinery on Wednesday morning was the second next year, and blasted a fireball into the neighbourhood. Ten houses suffered damage including cracked foundations, roofs lifted off, doors and windows blown out and debris hurled into rooms. One resident, Linda Wood, has been told to leave her house, which lies condemned after it was knocked clear of its foundations and damaged structurally.

Two local emergency dispatch centers were clogged up as calls came through to the 911 emergency number. Firefighters arrived quickly and tackled the resulting blaze at the refinery after establishing that there were no injuries. The 1950s refinery was not evacuated and is expected to continue operating despite the damage. It employs 80 people.

Silver Eagle’s own firefighting crews were already in control when they were joined by the emergency services, and together they put the flames out in around thirty minutes. Woods Cross mayor Kent Perry said that over the last six months there have been a lot of improvements in local firefighting and praised the reaction of emergency services. Later, 120 residents were visited by structural engineers to assess damage. There were also power outages in the area, with two other refineries amongst those affected.

Silver Eagle had also sent employees on a firefighting course following another explosion in January. In that accident, a large cloud of flammable vapour developed over a tank of naptha before igniting, causing a flash fire that injured several employees. The firm has said they will pay for all the damages caused by the latest incident, and Perry advised affected people to keep track of expenses incurred.

As well as the CSB, Utah’s Occupational Safety and Health Administration and Silver Eagle themselves are investigating the accident. The CSB released preliminary findings yesterday that indicated a burst pipe was to blame, although the reasons for its failure are unknown.

A pipe ten inches in diameter burst along a horizontal section near a point where it changed direction from vertical. The pipe normally carried hydrogen and diesel and was linked to a reactor that took waxes out of diesel fuel, but due to maintenance by Silver Eagle the pipe was only transporting pressurised hydrogen. When it failed at 9:15 630 pounds of hydrogen spilled out and were ignited by a nearby furnace. The resulting 800 degrees Fahrenheit fireball leapt 100 feet into the air and sent a string of pressure waves to buffet the housing, as the pipe pointed east towards the homes.

The CSB’s Donald Holmstrom commented that “The unit was immediately engulfed in flames… We’re extremely fortunate that no one got hurt. Five workers were in this process unit just a few minutes before.” The revelations come as local residents expressed concern about the fact that houses were built so close to the refinery in the first place.

It has been discovered that there were no houses near the refinery until six years ago, when an application was denied after an engineer commissioned by the council reported that the risks were too high. However, the developer convinced officials to change their minds with a report by a company he had hired himself. The CSB is due to look into these studies as well as one by the refinery. An opinion piece by the Salt Lake Tribune also criticised the company’s safety record, with the refinery also experiencing explosions in 2005 and 2007. However, the refinery had recently passed a safety inspection when the accident occurred. The CSB will examine whether the refinery was properly inspecting the pipe.

Monday, December 18, 2006

A state of emergency was declared Sunday for the U.S. state of Washington by governor Christine Gregoire, as additional reports of storm-related casualties surfaced. The state National Guard has been deployed to aid in distributing supplies.

Thousands were still without power in the coastal and Puget Sound regions, though most urban areas were back with power as late as Sunday afternoon, and outages were mostly contained to rural and unincorporated areas. Puget Sound Energy reported that roughly 500,000 energy customers out of the 700,000 who lost power were back in service by Sunday evening. Seattle City Light, the city’s independent municipal utility, reported only 18,000 customers still without power as of Monday morning, down from a peak of 175,000.

Four additional deaths related to the post-storm power outage had been reported as of Monday, bringing the total number of casualties to eight. A man in Gig Harbor was electrocuted by a downed power line while walking his dog. Another man in Spanaway died when an unattended candle caused a house fire.

Two died from carbon monoxide poisoning in separate incidents related to use of combustion devices indoors. Roughly a hundred additional cases of non-fatal carbon monoxide poisoning were reported from people using generators or grills indoors. News radio stations and authorities warned the public to stay away from downed power lines and not to use grills indoors. Dr. Neil Hampson at Virginia Mason’s hyperbaric unit, where a number of victims were being treated, warned it could be “the worse case of carbon monoxide poisioning in the country”.

On Monday, four new carbon-monoxide deaths were reported in a family of five in Burien due to an indoor generator. In Canada, which had some damage from the week’s storms, two southern British Columbia carbon monoxide deaths were also reported. Despite continued warnings, hospitals are still seeing cases of carbon monoxide poisoning, including a family in w:Shoreline, Washington which was taken to the hospital after they reported symptoms due to their indoor grill. Neighbors of the Burien family suggested that noise concerns are leading people to place noisy generators indoors.

The massive power outage left many stores and gas stations unable to operate. Some businesses opened with the help of backup generators, conserving power by foregoing heat and refrigeration, exterior lighting, and half the interior lighting. Most stores had run out of “D” size batteries, the most common size for flashlights, as well as firelogs and other essentials. Gasoline shortages were reported throughout the area, with one man selling excess fuel for as high as $15 per gallon, over 5 times the average retail price.

The Red Cross set up shelters throughout King and other affected counties for those without power or food. Hotels reported no vacancies as whole families took shelter in powered hotels, especially in Seattle. Restaurants also reported brisk business as people sought out a hot cooked meal. Tons of perishable food were expected to have become unsafe after the prolonged outage disabled refrigerators and freezers both in homes and stores.

Many of those without power visited nearby friends and family living where power had been continued or restored, while others traveled out of the area to places that had not been affected. The widespread outage made long-distance traveling treacherous on some major routes, with roadway lighting, cellular towers, and services disabled by the outage.

Most major roadways which were closed during the storm were reopened on Friday. The 520 Floating Bridge over Lake Washington, a major conduit to the technology-rich Eastside, sustained minor damage. Amtrak, which had halted its Cascades service, resumed Saturday evening. Sea-Tac Airport resumed operations with a reduced flight load, after a transient power outage on Friday disabled the airport radar and caused all planes to be grounded until it was repaired.

Thursday, July 28, 2005

Dr. Tewolde Berhan Gebre Egziabher, the Ethiopian government’s chief scientist and its representative to the Montreal-based UN Convention on Biological Diversity (CBD) had his passport returned without the requested Canadian visa, and without explanation. Dr. Tewolde is Africa’s chief negotiator for the Cartagena Protocol on Biosafety.

Tewolde had announced he would be visiting Montreal the first week of August to lobby for the labeling of genetically modified (GM) seeds and food products and urge companies and governments to accept liability for environmental contamination their seeds could lead to. Canada has yet to ratify the UN biosafety protocol and opposes compulsory genetically modified labeling and liability.

Dr. Tewolde has worked against terminator technology (genetically-modified seed rendered sterile at harvest time, forcing farmers to buy new seeds each growing season). In the past, Dr. Tewolde has disagreed with Canadian scientists on proposed methods to regulate GM products.

“Dr. Tewolde is one of the most respected scientists in his field,” said Pat Mooney, representative for the conservationist ETC Group in Ottawa, “If the Canadian government can’t make sure Dr. Tewolde has his visa for the opening of the meetings, Canada does not deserve to host the Convention on Biological Diversity.”

Dr. Tewolde himself is concerned that Canada’s actions signal a pattern, “Now that I have been prevented from coming to Montreal, who knows which ones of you will be prevented next time?” he wrote.

This closely mirrors another more recent incident where 12 Ugandans (mainly youths) were denied visas by the Canadian High Commission in Kenya. After making two applications and having met all the requirements asked by the commission, the Ugandan delegation was still denied visas.

Thursday, February 24, 2005

A paste containing synthetic tooth enamel can seal small cavities without drilling. Kazue Yamagishi and colleagues at the FAP Dental Institute in Tokyo say that the paste can repair small cavities in 15 minutes.

Currently, fillers don’t stick to such small cavities so dentists must drill bigger holes. Hydroxyapatite crystals, of which natural enamel is made, bond with teeth to repair tiny areas of damage.

Yamagishi and colleagues have tested their paste on a lower premolar tooth that showed early signs of decay. They found that the synthetic enamel merged with the natural enamel. The synthetic enamel also appears to make teeth stronger which will improve resistance to future decay. As with drilling, however, there is still the potential for pain: The paste is strongly acidic to encourage crystal growth and causes inflammation if it touches the gums.

The paste is reported in the journal Nature.

Thursday, June 30, 2005

Seoul —

The OhmyNews International Citizen Reporters’ Forum (see previous report) concluded on Saturday with several speeches and a visit to sponsoring companies in South Korea’s technology sector. Some invited “citizen reporters” from around the world extended their stay by several days to tour the country. Since the conclusion of the conference, presentations and transcripts have been published on the OhmyNews web site. [1]

Wednesday, October 17, 2007

Vernon Bellecourt, once the primary spokesperson for the American Indian Movement, died recently at age 75. Bellecourt, an Ojibwa who fought for Native rights, was perhaps best known for his opposition to Native names and mascots for sports teams.

First in the headlines in 1972, Bellecourt organized a cross-country caravan of the Movement, to Washington. Once there, members of the group occupied the Bureau of Indian Affairs offices. His goal of international recognition for Aboriginal nations and their treaties found him meeting with figures like Libyan Colonel Muammar el-Qaddafi, and Palestine’s Yasir Arafat. In 1977 Leonard Peltier was convicted and sentenced to two consecutive life terms for the murder of two FBI Agents during a 1975 shoot-out on the Pine Ridge Indian Reservation; Bellecourt led the campaign to free him.

Most recently, he visited Venezuelan President Hugo Chávez, to discuss getting free or cheap heating oil for reservations.

His work as president of the National Coalition on Racism in Sports and Media made a much wider known mark, though. Bellecourt emphasized that he believed such names perpetuated racial stereotypes, clouding the real identities and problems facing natives.

Teams with native-related names could almost guarantee on Bellecourt showing up at major games. He twice burned an effigy of Chief Wahoo, the Cleveland Indians baseball team mascot, and both times was arrested. When the Washington Redskins of the National Football League made the Super Bowl, Vernon was there to protest. The United States Commission on Civil Rights was critical of such names by 2001, calling them “insensitive in light of the long history of forced assimilation”. Some newspapers have stopped using the names of teams with Native origins.

None of his “big four” targets have shown any indication of changing: the Washington Redskins, the Kansas City Chiefs, the Cleveland Indians or the Atlanta Braves.

Post-season use of American Indian mascots were banned by the National Collegiate Athletic Association in 2005, suggesting the names are “hostile or abusive”. Bellecourt was pleased with the NCAA sanctions, but suggested such actions were only going “half way”.

The Florida State Seminole and the Illinois Illini were among the 18 colleges affected by the ban. Florida president T.K. Wetherell threatened legal action in response. The Florida Seminole tribes have endorsed the University’s usage of the name, but some out-of-state tribes were “not supportive”, according to the NCAA vice president for diversity and inclusion.

Born WaBun-Inini, Bellecourt died from complications of pneumonia on October 13, in Minneapolis, Minnesota, United States.